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er skbr3 human bc cell lines  (ATCC)


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    ATCC er skbr3 human bc cell lines
    Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and <t>SKBR3</t> BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.
    Er Skbr3 Human Bc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Keratin 24 Predicts Poor Prognosis in Breast Cancer"

    Article Title: Keratin 24 Predicts Poor Prognosis in Breast Cancer

    Journal: Pathobiology

    doi: 10.1159/000546022

    Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and SKBR3 BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.
    Figure Legend Snippet: Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and SKBR3 BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.

    Techniques Used: Western Blot, Immunohistochemical staining, Expressing, Staining, Membrane, In Situ



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    Image Search Results


    Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and SKBR3 BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.

    Journal: Pathobiology

    Article Title: Keratin 24 Predicts Poor Prognosis in Breast Cancer

    doi: 10.1159/000546022

    Figure Lengend Snippet: Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and SKBR3 BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.

    Article Snippet: To validate the specificity of KRT24, both luminal A type (MCF7) and HER2-positive ER− (SKBR3) human BC cell lines (obtained from the American Type Culture Collection, Rockville, MD, USA) were used, as recommended by the manufacturer.

    Techniques: Western Blot, Immunohistochemical staining, Expressing, Staining, Membrane, In Situ

    Representative confocal microscopy of ( A ) SKBR3 and ( B ) HDFa (scale bar = 20 μm) incubated for 60 min with NR-LLNCs, NR-LLNCs-HER2, and NR-LLNCs-HER2-PC. Red filter and green filter correspond to LLNCs labeled with Nile Red and LLNCs labeled with HER2-FITC, respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Effect of the Protein Corona Formation on Antibody Functionalized Liquid Lipid Nanocarriers

    doi: 10.3390/ijms242316759

    Figure Lengend Snippet: Representative confocal microscopy of ( A ) SKBR3 and ( B ) HDFa (scale bar = 20 μm) incubated for 60 min with NR-LLNCs, NR-LLNCs-HER2, and NR-LLNCs-HER2-PC. Red filter and green filter correspond to LLNCs labeled with Nile Red and LLNCs labeled with HER2-FITC, respectively.

    Article Snippet: The SKBR3 human breast cancer (BC) cell line was obtained from American Type Culture Collection (ATCC) and cultured following ATCC recommendations.

    Techniques: Confocal Microscopy, Incubation, Labeling

    In vitro uptake of NR-LLNCs, NR-LLNCs-HER2, and NR-LLNCs-HER2-PC in both SKBR3 and HDFa analyzed by flow cytometry. Values represent the fluorescence intensity of the merge channels for green and red as NR-LLNCs-HER2 are both red and green fluorescent. Data are mean values ± SD. * p < 0.001 shows the significant values calculated using t -test.

    Journal: International Journal of Molecular Sciences

    Article Title: Effect of the Protein Corona Formation on Antibody Functionalized Liquid Lipid Nanocarriers

    doi: 10.3390/ijms242316759

    Figure Lengend Snippet: In vitro uptake of NR-LLNCs, NR-LLNCs-HER2, and NR-LLNCs-HER2-PC in both SKBR3 and HDFa analyzed by flow cytometry. Values represent the fluorescence intensity of the merge channels for green and red as NR-LLNCs-HER2 are both red and green fluorescent. Data are mean values ± SD. * p < 0.001 shows the significant values calculated using t -test.

    Article Snippet: The SKBR3 human breast cancer (BC) cell line was obtained from American Type Culture Collection (ATCC) and cultured following ATCC recommendations.

    Techniques: In Vitro, Flow Cytometry, Fluorescence